本文以反相高效液相色谱法测定人血浆中尼莫地平的浓度。以尼群地平和甲基睾丸素为内标物，碱性条件下用乙醚－正已烷（１：１）萃取，流动相为甲醇－水－正丁胺（６５：３５：０，００１），流速１．２ｍｌ／ｍｉｎ，紫外检测波长２３８ｎｍ，线性范围５～１００ｎｇ／ｍｌ，最低检测浓度２ｎｇ／ｍｌ，日内差异小于５％，日间差异约为５％。６名健康受试者口服－种国产尼莫地平片剂后，以本方法测定其体内药代动力学符合一室模型。 This article uses reversed-phase high performance liquid chromatography to determine the concentration of nimodipine in human plasma. Nitrendipine and methyltestosterone were used as internal standard and extracted with ether-n-hexane (1: 1) under basic condition. The mobile phase consisted of methanol-water-n-butylamine (65: 35: 0,001) , The flow rate of 1.2ml / min, UV detection wavelength of 238nm, linear range of 5 ~ 100ng / ml, the lowest detection concentration of 2ng / ml, intraday difference of less than 5%, day difference of about 5%. After oral administration of a domestic nimodipine tablet to six healthy subjects, the in vivo pharmacokinetics of the method was in accordance with the one-compartment model.