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应用乙型肝炎核心抗原(HBcAg)对其相应的抗体(抗HBc)及抗HBcIgM的检测,对乙型肝炎的临床诊断、分型、流行病学调查以及对乙肝疫苗安全性鉴定等都具有重要意义。但因HBcAg来自乙肝病人的尸肝或富含Dane颗粒的病人血清中,因此来源困难,不能大量生产。1981年以来,国外报导了应用基因工程技术在大肠杆菌中合成HBcAg来检测抗HBc,取得较好结果。我们实验室于1982年构建了adw亚型HBV无性繁殖系。随后采用bla启动子使HBcAg在大肠杆菌中获得表达,并以这种抗原用ELISA方法检测乙肝病人血清中抗-HBc和抗HBcIgM,获得比较满意的效果。在此基础上,应用基因工程技术构建更高表达HBcAg的工程菌株,本文报告采用γ噬菌体PL启动子,使HBc基因在大肠杆菌中表达。
The application of hepatitis B core antigen (HBcAg) to the detection of its corresponding antibodies (anti-HBc) and anti-HBcIgM is important for the clinical diagnosis, typing, epidemiological investigation of hepatitis B and the safety of hepatitis B vaccine significance. However, because HBcAg comes from the dead liver of patients with hepatitis B or from the serum of patients with Dane granules, it is difficult to source and can not be mass-produced. Since 1981, foreign reports of the application of genetic engineering in the synthesis of HBcAg in E. coli to detect anti-HBc, and achieved good results. Our laboratory constructed the adw subtype HBV asexual propagation line in 1982. Subsequently, the bla promoter was used to express HBcAg in E. coli, and the anti-HBc and anti-HBcIgM in the serum of patients with hepatitis B were detected by this antigen using the ELISA method, and satisfactory results were obtained. On this basis, the use of genetic engineering technology to build HBcAg-engineered strains higher, the report reported the use of gamma phage PL promoter, HBc gene expression in E. coli.