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用0.7%琼脂糖凝胶电泳检查了自1996年以来云南南部及东南部所分离的41株鼠疫菌质粒DNA,结果表明来自石屏、文山、砚山、建水、个旧、禄春、开远7个县(市)的菌株质粒图谱同来自澜沧江下游的澜沧、临沧、勐海、景洪及元江流域的思茅地区的菌株一样,其中石屏分离4株缺失6Mdal质粒带型,但出现—分子量约12Mdal的清晰带型,用Pla基因作为引物,做PCR分析结果为阳性,推测可能为6Mdal质粒的二聚体。结合流行病学分析认为滇南、滇东南部分地区可能同属一片鼠疫自然疫源地。
The isolates of 41 Y. pestis isolates from southern Yunnan and southeastern Yunnan since 1996 were analyzed by 0.7% agarose gel electrophoresis. The results showed that the isolates from 41 strains of Yersinia pestis from Shi Ping, Wenshan, Yanshan, Jianshui, Gejiu, Luchun and Kaiyuan The isolates from 7 counties (cities) were the same as the strains from Lancang, Lincang, Menghai, Jinghong and Simao areas in the lower reaches of the Lancang River. However, there were 4 isolates with 6Mdal deletion but no - A clear band pattern with a molecular weight of about 12Mdal. Using the Pla gene as a primer, the result of PCR analysis was positive, presumably a dimer of 6Mdal plasmid. Combined with epidemiological analysis, it is thought that some areas in southern Yunnan and southeast Yunnan may belong to a natural epidemic of plague.