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对１５例良性前列腺增生（ＢＰＨ）和１２例正常前列腺组织标本中胰岛素样生长因子－Ｉ－（ＩＧＦ－Ｉ）ｍＲＮＡ的表达情况进行了研究。ＲＴ－ＰＣＲ的方法可自所有标本中检出ＩＧＦ－ＩｍＲＮＡ的表达，并用电泳和Ｓｏｕｔｈｅｒｎ杂交证实了结果的可靠性。原位杂交表明１３／１５的ＢＰＨ和９／１２的正常前列腺标本中有ＩＧＦ－ＩｍＲＮＡ表达，ＩＧＦ－Ｉ在上皮的表达强于间质，尤以基底细胞表达最明显。ＩＧＦ－ＩｍＲＮＡ在ＢＰＨ组织中的量多于正常前列腺。
The expression of insulin-like growth factor-I (IGF-I) mRNA in 15 cases of benign prostatic hyperplasia (BPH) and 12 cases of normal prostatic tissue was studied. RT-PCR was used to detect the expression of IGF-1 mRNA in all specimens, and the reliability of the results was confirmed by electrophoresis and Southern blotting. In situ hybridization showed that IGF-I mRNA was expressed in 13/15 BPH and 9/12 normal prostate specimens. The expression of IGF-I in the epithelium was stronger than that in the stroma, especially in basal cells. IGF-I mRNA is present in BPH tissue more often than normal prostate.