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为从蛋白组学水平揭示不同抗性小麦品种对条锈病的响应机制,以3个不同抗性小麦品种为材料,从蛋白质组调控的角度分析了条锈菌侵染后不同抗性小麦品种叶片蛋白质的表达差异。结果显示,侵染菌种CY32与混合菌后,高抗条锈病品系天9524、慢条锈病品种兰天15号和高感条锈病品种铭贤169叶片中一些蛋白的表达呈现出显著上调或下调,利用MALDI-TOF/TOF MS/MS质谱鉴定和MASCOT数据库检索,从3个小麦品种中共注释出16个差异表达蛋白点,其中铭贤169中7个,兰天15号中6个,天9524中3个。按照其功能分类,这些差异表达蛋白点分别参与了叶绿体光合作用,蛋白质合成,抗病响应等生理活动。这些差异表达蛋白可能与小麦响应条锈菌侵染过程有关,为抗条锈病相关基因的克隆与抗病响应机制的研究提供参考。
In order to reveal the response mechanism of different resistant wheat cultivars to stripe rust from proteomics level, three different resistant wheat cultivars were used as materials to analyze the effects of stripe rust on the leaves of different resistant wheat cultivars Differences in protein expression. The results showed that the expression of some proteins in the stripe rust resistant strain 9524, the stripe rust resistant variety Lantian 15 and the high susceptible stripe rust cultivar Mingxian 169 showed significant up-regulation or down-regulation , 16 differentially expressed protein spots were annotated from 3 wheat cultivars by using MALDI-TOF / TOF MS / MS mass spectrometry and MASCOT database search. Among them, 7 were identified in Mingxian 169, 6 in Lantian 15 and 9524 In 3. According to their functional classification, these differentially expressed proteins were involved in the physiological activities of chloroplast photosynthesis, protein synthesis, disease resistance response, respectively. These differentially expressed proteins may be related to the wheat response to stripe rust infection and provide reference for the study of the cloning and disease resistance response genes related to stripe rust resistance.