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目的建立黄连及其炮制品和黄连须的薄层鉴别方法。方法采用二次展开薄层色谱法。结果在同一薄层板上以环己烷-乙酸乙酯-异丙醇-乙腈-水-三乙胺(2.5∶3∶1∶2∶0.5∶1)第一次展开、乙酸乙酯-乙腈-浓氨水(2∶7∶1)第二次展开,薄层色谱的斑点清晰,重复性好。结论所用方法专属性及重复性均较好,可为2015年版《中国药典》黄连须质量标准中的薄层鉴别方法的建立奠定基础。
OBJECTIVE To establish a TLC identification method of Coptis chinensis and its processed products and Coptis chinesis. Methods Two-fold thin-layer chromatography was used. The results were first developed on the same plate with cyclohexane-ethyl acetate-isopropanol-acetonitrile-water-triethylamine (2.5: 3: 1: 2: 0.5: 1), ethyl acetate- - concentrated ammonia (2: 7: 1) second expansion, TLC spots clear, good repeatability. Conclusions The method used has good specificity and repeatability, which lays the foundation for the establishment of thin layer identification method in the Chinese Pharmacopoeia Coptis in the 2015 edition.