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In this paper,we developed and validated a simple,sensitive,and selective high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) method to identify and measure the following prohibited substances that may be found in cosmetic products:minoxidil,hydrocortisone, spironolactone,estrone,canrenone,triamcinolone acetonide and progesterone.Chromatographic separation was performed on a Waters Symmetry C18(100 mm×2.1 mm,3.5μm particle size) with a gradient elution system composed of 0.2%(v/v) formic acid aqueous solution and methanol containing 0.2%(v/v) formic acid at a flow rate of 0.3 mL/min.The substances were detected using a triple quadrupole mass spectrometer in the multiple reaction monitoring mode with an electrospray ionization source.All of the calibration curves showed good linearity(r > 0.999) within the tested concentration ranges.The limit of detection was <25 pg.The relative standard deviations for intraday precision for each of the prohibited substances were <3.5%at two concentration levels(2μg/g,10μg/g). The relative recovery rate for each of the prohibited substances ranged from 91.8%to 111%at three concentration levels(0.1μg/g,2μg/g,10μg/g),including the limit of quantification.In conclusion,we have developed and validated a method that can identify seven prohibited substances in cosmetic products.
In this paper, we developed and validated a simple, sensitive, and selective high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS / MS) method to identify and measure the following prohibited substances that may be found in cosmetic products: minoxidil, hydrocortisone, spironolactone, estrone, canrenone, triamcinolone acetonide and progesterone. Chromatographic separation was performed on a Waters Symmetry C18 (100 mm × 2.1 mm, 3.5 μm particle size) with a gradient elution system composed of 0.2% (v / v) aqueous solution and methanol containing 0.2% (v / v) formic acid at a flow rate of 0.3 mL / min. The substances were detected using a triple quadrupole mass spectrometer in the multiple reaction monitoring mode with an electrospray ionization source. All of the calibration curves showed good linearity (r> 0.999) within the tested concentration range. The limit of detection was <25 pg. the relative standard deviations for intraday precision for each of the prohibited substances w ere <3.5% at two concentration levels (2 μg / g, 10 μg / g) The relative recovery rate for each of the prohibited substances ranged from 91.8% to 111% at three concentration levels (0.1 μg / g, / g), including the limit of quantification. conclusion, we have developed and validated a method that can identify seven prohibited substances in cosmetic products.