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目的观察复方瑞康欣(CR)对吗啡戒断焦虑大鼠海马、杏仁核和伏核突触形态结构可塑性的影响。方法雄性SD大鼠84只,随机分为生理盐水对照组、吗啡戒断焦虑模型组、CR高、中、低剂量组和丁螺环酮组。以剂量递增方式皮下注射吗啡,10 d后自然戒断,于戒断1～3 d CR灌胃治疗后行高架十字迷宫实验。采用透射电镜技术比较各组(6只)突触体视学和界面结构各参数。结果在300 mg/kg和200 mg/kg CR组,大鼠进入开放臂次数的百分比和在开放臂停留时间的百分比明显增加(P<0.01或P<0.05);海马、杏仁核突触的数密度和面密度显著降低(P<0.01),突触连接带平均面积增加(P<0.01或P<0.05),伏核突触数密度降低(P<0.01);海马突触后致密物厚度、突触活性区长度、间隙宽度和界面曲率降低(P<0.01或P<0.05)。结论 CR能缓解吗啡依赖戒断所引起的焦虑,其细胞生物学机制可能是逆转吗啡戒断时海马、杏仁核和伏核突触形态结构的可塑性。
Objective To observe the effect of Fufang Ruikangxin on morphological plasticity of hippocampus, amygdala and nucleus accumbens in anxious rats with morphine withdrawal. Methods Eighty-four male Sprague-Dawley rats were randomly divided into normal saline control group, morphine withdrawal anxiety model group, CR high, medium and low dose groups and buspirone group. Morphine was subcutaneously injected in a dose-escalation manner. After 10 days, morphine was withdrawn spontaneously and elevated maze test was performed after abstinence for 1-3 days. Transmission electron microscope was used to compare the parameters of visual acuity and interface structure in each group (6). Results In the 300 mg / kg and 200 mg / kg CR groups, the percentages of rats entering the open arms and the percentages of staying in the open arms were significantly increased (P <0.01 or P <0.05). The number of hippocampus and amygdala synapses (P <0.01 or P <0.01), the average area of synaptic junction increased (P <0.01 or P <0.05), the number density of nucleus accumbens decreased (P <0.01) Synaptic active region length, gap width and interface curvature decreased (P <0.01 or P <0.05). Conclusion CR can relieve the anxiety caused by morphine withdrawal and its cellular mechanism may be to reverse the morphological plasticity of hippocampus, amygdala and nucleus accumbens in morphine withdrawal.