论文部分内容阅读
目的丁苯酞预处理对大鼠脑缺血再灌注损伤后脑水肿、血脑屏障和基质金属蛋白酶-9(MMP-9)表达的影响。方法选取48只健康雄性大鼠,随机分为假手术组(Sham,16只),缺血再灌注组(I/R,16只)和丁苯酞干预组(TanⅡA,16只),用线栓法阻塞大鼠右大脑中动脉制作局灶性脑缺血再灌注模型。干湿重法测定脑含水量反映脑水肿程度,比色法测定脑组织伊文思蓝(EB)含量反映血脑屏障的损伤程度,免疫组织化学方法法和Western blot方法检测各组大鼠脑组织MMP-9蛋白表达,Real-time PCR检测MMP-9m RNA表达。结果相比于假手术组,缺血再灌注组大鼠脑含水量及EB含量显著升高,而NBP预处理组脑含水量及EB含量明显降低(<0.01)。相比于假手术组,缺血再灌注组大鼠脑组织MMP-9蛋白及及m RNA的表达水平均显著升高,NBP预处理组则显著降低(<0.01)。结论 NBP预处理对大鼠脑缺血再灌注损伤的保护作用,可能与调节MMP-9的表达、降低血脑屏障通透性和减轻脑水肿相关。
Effect of Butylphthalide Preconditioning on Cerebral Edema, Blood Brain Barrier and Expression of Matrix Metalloproteinase -9 in Rats with Cerebral Ischemia / Reperfusion Injury. Methods Forty-eight healthy male rats were randomly divided into sham operation group (Sham 16), ischemia reperfusion group (I / R 16) and butylphthalide intervention group (Tan Ⅱ A, 16 rats) Obstruction of middle cerebral artery of right middle cerebral artery occlusion rats to make focal cerebral ischemia reperfusion model. The brain water content was measured by dry-wet method to reflect the degree of brain edema. The content of Evans blue (EB) in brain tissue was measured by colorimetric method to reflect the degree of blood-brain barrier injury. Immunohistochemistry and Western blot were used to detect the brain tissue MMP-9 protein expression, Real-time PCR detection of MMP-9mRNA expression. Results Compared with sham operation group, the content of water and EB in brain of ischemia-reperfusion group was significantly increased, while the content of brain water and EB in NBP preconditioning group were significantly decreased (P <0.01). Compared with sham operation group, the expression of MMP-9 protein and m RNA in brain tissue of ischemia-reperfusion group were significantly increased, while NBP preconditioning group was significantly lower (P <0.01). Conclusions NBP preconditioning may have protective effects on cerebral ischemia-reperfusion injury in rats, which may be related to the regulation of MMP-9 expression, the decrease of BBB permeability and the alleviation of cerebral edema.