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基因重组E1缺陷腺病毒可作为基因治疗的载体,但仅删除E1并不能完全阻断病毒基因的表达。病毒蛋白质可激活特异性CTL,导致外源基因表达逐渐减少。为获得更理想的可用于基因治疗的稳定缺陷腺病毒载体,本文比较了一组插入大肠杆菌lacZ基因、表达受RSV LTR控制的E1和E1/E4缺陷腺病毒在体外和体内的生物学特性。作者构建了5种重组腺病毒:AdE3+βGal为E1缺陷,余4种为E1/E4缺陷病毒;AdβGal/dl1004、AdβGal/dl1007、AdβGal/dl1011和AdβGal/dl1014,其E4有不同程度缺陷。每种重组的腺病毒均有完整的E3,并
Recombinant E1-deficient adenovirus can serve as a gene therapy vector, but deletion of E1 alone does not completely block the expression of the viral gene. Viral proteins activate specific CTLs resulting in a gradual reduction of foreign gene expression. In order to obtain a more desirable stable adenovirus vector for gene therapy, a set of in vitro and in vivo biological characteristics of E1 and E1 / E4-deficient adenovirus expressing E. coli lacZ gene and expressing RSV LTR were compared. The authors constructed five kinds of recombinant adenovirus: AdE3 + βGal is E1 deficiency, the remaining four are E1 / E4 deficiency virus; AdβGal / dl1004, AdβGal / dl1007, AdβGal / dl1011 and AdβGal / dl1014, E4 have different degrees of defects. Each recombinant adenovirus has intact E3, and