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我们应用含小鼠B71、B72基因片段的真核表达载体PCI neo B71和PCI neo B72分别转染H22肝癌细胞,探讨B71、B72在肿瘤免疫过程中的不同功能与作用。一、材料与方法1.实验动物:雌性BALB/C小鼠,8～12周龄,购自武汉华中科技大学实验动物中心。实验分组:A组为H22/Wt组;B组为H22/neo组
We used mouse neoplasia B71 and B72 gene eukaryotic expression vector PCI neo B71 and PCI neo B72 were transfected H22 hepatocellular carcinoma cells to explore the different functions of B71 and B72 in tumor immunity. Materials and Methods 1. Experimental animals: Female BALB / C mice, 8-12 weeks old, purchased from Laboratory Animal Center, Huazhong University of Science and Technology, Wuhan. Experimental grouping: group A was H22 / Wt group; group B was H22 / neo group