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常规培养阴道滴虫的培养基,需用动物血清我们自制了一种鸡胚汤培养基,比阴道分泌物直接涂片镜检阳性率高15%.且方法简便,价格低廉,适合于一时得不到动物血清的单位使用.现予介绍.1.鸡胚汤培养基的制备取培养12天的鸡胚(去嘴、眼)并用剪刀剪碎称取20g,置100ml 蒸馏水中浸制1h(摇动3次),水浴煮沸5min,用脱脂棉过滤,加水补足100ml,加蛋白胨2g,葡萄糖1g,麦芽糖1g,氯化钠0.5g,再加热至溶解,冷却后用滤纸过滤即为鸡胚汤培养基.调整pH5.6～5.8,然后分
Conventional culture of trichomonas vaginalis medium, the need for animal serum we made a chicken embryo soup medium, direct smear than vaginal secretions microscopy positive rate of 15%. And the method is simple, inexpensive, suitable for a moment Less than animal serum units are introduced.Preliminary preparation of chicken embryo soup medium Take 12 days of culture of chicken embryos (mouth, eyes) and scissors scissors weighed 20g, set in 100ml of distilled water immersion 1h Shake 3 times), the water bath to boil 5min, with cotton wool filter, add water to make up 100ml, add peptone 2g, glucose 1g, maltose 1g, sodium chloride 0.5g, and then heated to dissolve, filtered with filter paper after cooling is chicken embryo soup medium Adjust pH5.6 ~ 5.8, then divide