目的:建立同时测定排石颗粒中迷迭香酸、当药苷和马钱苷含量HPLC法。方法:采用Agilent Epclipse plus C18色谱柱(4.6 mm×250 mm,5μm);以0.1%磷酸溶液为流动相A,甲醇:乙腈(2:1)为流动相B进行梯度洗脱;流速1.00 mL/min,检测波长240 nm。结果:迷迭香酸、当药苷和马钱苷分别在0.2008~4.016μg、0.067~1.34μg、0.04818~0.9636μg范围内呈现良好的线性关系(r≥0.9999)。平均回收率≥98.8%,RSD≤2.1%(n=6)。结论:该方法准确、简便、可靠,重复性好,可用于本品的质量控制。 OBJECTIVE: To establish a simultaneous determination of rosmarinic acid in paeonite granules by HPLC method. METHODS: The mobile phase A was eluted with 0.1% phosphoric acid solution and methanol: acetonitrile (2: 1) as mobile phase B using an Agilent Epclipse plus C18 column (4.6 mm × 250 mm, 5 μm) min, detection wavelength 240 nm. Results: Rosmarinic acid, when the glycosides and loganin in the range of 0.2008 ~ 4.016μg, 0.067 ~ 1.34μg, 0.04818 ~ 0.9636μg showed a good linear relationship (r ≥ 0.999). The average recovery was ≥98.8%, RSD ≤ 2.1% (n = 6). Conclusion: The method is accurate, simple, reliable, reproducible, and can be used for the quality control of this product.