运用MTT法测定胃癌体外对化疗药物的敏感性及其临床适用价值的评估

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目前已建立的体外抗癌药物敏感试验的方法有多种,但Mosman[1]建立的MTT法具有其它方法不可比拟的优点,其原理是活细胞内线粒体脱氨酶能将四氨咄化合物,由黄色裂解为蓝色甲联,并且甲服生成量与活细胞数成正比[2,3J。本研究用MTT法预测胃癌体外化疗药物的敏感性,为临床选 There are many methods for the in vitro anticancer drug sensitivity test that have been established, but the MTT method established by Mosman [1] has advantages that are incomparable to other methods. The principle is that the mitochondrial deaminase in living cells can bind tetraammine compounds. Cleavage from yellow to blue gamma, and the amount of production is proportional to the number of viable cells [2, 3J. In this study, MTT assay was used to predict the sensitivity of gastric cancer chemotherapy drugs in vitro.
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