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为了探究黄芩素是否具有抗衰老作用,本研究以果蝇为模式生物,考察在自然衰老模型、过氧化氢损伤模型和百草枯损伤模型中黄芩素对雌性果蝇寿命的影响。在此基础上,采用生物信息学方法预测黄芩素发挥抗衰老活性可能作用的靶点,并对靶点进行通路富集,选择影响较大的氧化应激通路进行实验验证。结果表明,0.04和0.2 mg·m L-1黄芩素均能延长自然衰老模型中果蝇的平均寿命和最高寿命,并可有效减弱由过氧化氢和百草枯引起的果蝇寿命缩短。靶点预测结果显示黄芩素可能作用于31个衰老相关靶点,并能显著影响氧化应激通路,进一步实验结果表明黄芩素可能通过降低果蝇体内活性氧(reactive oxygen species,ROS)、丙二醛(malondialdehyde,MDA)、氧化型谷胱甘肽(oxidized glutathione,GSSG)含量,从而减轻氧化应激水平,延缓衰老。
In order to explore whether baicalein has anti-aging effects, we studied the effects of baicalein on the longevity of female flies in natural aging model, hydrogen peroxide injury model and paraquat-induced injury model using fruit fly as a model organism. On this basis, the bioinformatics method is used to predict the potential role of baicalein in anti-aging activity, and the pathway is enriched for the target site, and the oxidative stress pathway with greater influence is selected for experimental verification. The results showed that both 0.04 and 0.2 mg · mL -1 baicalein could prolong the average lifespan and maximum lifespan of fruit flies in the natural aging model and effectively reduce the shortened lifespan of fruit flies caused by hydrogen peroxide and paraquat. The results of target prediction showed that baicalein could act on 31 senescence-related targets and significantly affect the oxidative stress pathway. Further experiments showed that baicalein could reduce reactive oxygen species (ROS) Malondialdehyde (MDA) and oxidized glutathione (GSSG), so as to reduce the level of oxidative stress and delay the aging.