长泰砂仁花粉活力的初步观察

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1、试验证明长泰砂仁花粉粒发育不受年龄大小及阴蔽度强弱影响,花粉粒发育状况都很正常,一般萌发率高达86.59%—99.08%。当前生产上存在结果率低与花粉萌发率似无关系。 2、砂仁花粉粒在气温25℃条件下,置于15%蔗糖加0.0005%硼酸的培养基中,培养10分钟花粉粒开始萌发,2小时花粉管伸长至互相交义,花粉管末端由尖型膨大成圆形,并有黄色稠浓的内含物,标志着花粉管的胞壁将破裂进入授精阶段,花粉粒已几乎全部萌发,萌发率达99.06%。 3、在15%蔗糖浓度下加少量硼酸,可刺激花粉粒萌发。因此在砂仁开花前后喷施适当硼酸及蔗糖是有利于受精及提高结果率。 4、砂仁花粉粒萌发率与花粉粒的成熟度关系极大,已成熟的花粉粒萌发率可达90.48%,比未成熟的高11.67倍。试验证实上午9时以后散粉盛期花药完全爆裂的花粉粒已充分成熟,可以做为人工杂交育种授粉材料。 5、砂仁花粉粒的萌发力与贮存时间成反比,贮存时间越长萌发力越低。但从花药完全爆裂到第二天13时30分,萌发率还可保持在75%。说明仍有传粉受精作用。 1, The experiment proves that the growth of pollen grains of Changteng Amomum is not affected by the age and the degree of shade degree. The development of pollen grains is normal, with the germination rate as high as 86.59% -99.08%. The current low production rate of production and pollen germination rate seems unrelated. 2. Amomum pollen grains were placed in medium of 15% sucrose plus 0.0005% boric acid at a temperature of 25 ° C. The pollen grains started to germinate after culturing for 10 minutes, the pollen tubes extended to mutual contact after 2 hours, Pointed swollen into a round, and the yellow thick content, indicating that the cell wall of the pollen tube will burst into the stage of insemination, pollen grains have been almost all germination, germination rate of 99.06%. 3, 15% sucrose concentration plus a small amount of boric acid can stimulate pollen germination. Therefore, spraying Amomum villosum before and after spraying appropriate boric acid and sucrose is conducive to fertilization and improve the rate of results. The germination rate of pollen grains of Amomum villosa is very much related to the maturity of pollen grains. The mature pollen germination rate reaches 90.48%, which is 11.67 times higher than that of immature ones. The experiment confirmed that pollen grains completely burst after anthesis at 9 am have been fully ripe and can be used as artificial hybrid breeding pollination materials. 5, Amomum villosum germination force and storage time is inversely proportional to the storage time, the lower the germination force. However, the germination rate could remain at 75% after the anthers completely burst to 13:30 the next day. Explain that there is still pollination and fertilization.
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