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应用ＰＣＲ－ＳＳＣＰ方法对２４例血清ＨＢｓＡｇ（＋）患者肝癌及癌旁组织中Ｐ５３和Ｋ－ｒａｓ基因突变进行了检测。肝癌组织中Ｐ５３基因突变率为３３％，Ｋ－ｒａｓ基因突变率为２５％，癌旁正常组织中无Ｐ５３和Ｋ－ｒａｓ基因突变，癌旁硬化组织中Ｋ－ｒａｓ突变为１５％，无Ｐ５３基因突变。在有ＨＢＶ的肝场组织中Ｐ５３基因突变率为１００％，Ｋ－ｒａｓ突变率为６７％，提示Ｐ５３与Ｋ－ｒａｓ基因突变机制与ＨＢＶ感染有一定关系。Ｐ５３与Ｋ－ｒａｓ突变无相关性。后者可能是肝癌形成过程中的早期行为，前者与肝癌的发展、进行有关。
The PCR-SSCP method was used to detect the mutations of P53 and K-ras genes in 24 cases of serum HBsAg(+) patients. The mutation rate of P53 gene in hepatocellular carcinoma was 33%, the K-ras gene mutation rate was 25%, no P53 and K-ras gene mutations were found in para-tumor normal tissues, K-ras mutation in paraneoplastic tissues was 15%, no P53. Gene mutation. The mutation rate of P53 gene in hepatic tissues with HBV is 100%, and the mutation rate of K-ras is 67%. This suggests that the mutation mechanism of P53 and K-ras has a certain relationship with HBV infection. There was no correlation between P53 and K-ras mutations. The latter may be the early behavior in the process of liver cancer formation, and the former is related to the development and progression of liver cancer.