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目的研究5-氮-2-脱氧胞苷(5-Aza-Cdr)和曲古抑菌素 A(TSA)在体内、体外对人胆管癌细胞 QBC-939生长的影响,探讨其应用于胆管癌生物学治疗的价值。方法应用生长曲线、MTT、流式细胞仪、胆管癌裸鼠种植模型,检测不同浓度的5-Aza-Cdr 和 TSA,以及联合化疗药物对QBC-939体内、体外增殖的影响。结果 5-Aza-Cdr 和 TSA 对 QBC-939有明显的抑制作用,呈浓度、时间依赖性。流式细胞仪检测细胞周期主要阻滞于 G_1/S 期,凋亡不明显。QBC-939经处理后裸鼠体内种植瘤生成率降低,荷瘤小鼠给予5-Aza-Cdr,TSA 和氟尿嘧啶后肿瘤生长速度减慢、部分体积减小。结论 5-Aza-Cdr 和 TSA 在体内、体外能抑制人胆管癌细胞 QBC-939的生长,可能为胆管癌的生物学治疗提供一种新的思路。
Objective To investigate the effects of 5-Aza-Cdr and trichostatin A (TSA) on the growth of human cholangiocarcinoma cell line QBC-939 in vitro and in vivo. The value of biological treatment. Methods Growth curves, MTT, flow cytometry and cholangiocarcinoma in nude mice models were used to detect the effects of 5-Aza-Cdr and TSA in different concentrations on the proliferation of QBC-939 in vitro and in vivo. Results 5-Aza-Cdr and TSA significantly inhibited QBC-939 in a concentration-and time-dependent manner. Flow cytometry cell cycle arrest in the G_1 / S phase, apoptosis was not obvious. After treated with QBC-939, the incidence of implanted tumors in nude mice decreased. The tumor growth rate of 5-Aza-Cdr, TSA and 5-Fluorouracil in tumor-bearing mice slowed down and some of the volume decreased. Conclusion 5-Aza-Cdr and TSA can inhibit the growth of human cholangiocarcinoma cells QBC-939 in vivo and in vitro, which may provide a new idea for the biological treatment of cholangiocarcinoma.