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目的:优化筛选大孔树脂富集纯化牛蒡子中木脂素的工艺条件。方法:以牛蒡苷、牛蒡苷元的比吸附量、比解吸量、吸附率、解吸率为指标,采用静态吸附-解吸试验,对比5种树脂的纯化效果;通过静态吸附-解吸附动力学曲线,确定吸附和解吸时间;通过单因素考察,确定解吸剂浓度、上样液浓度、流速等相关参数。结果:D-101型树脂对牛蒡子中木脂素的吸附和解吸性能较好,根据试验结果并结合生产实际,确定工艺条件为:上样液浓度以牛蒡苷计为4.5~6.0 mg/m L,树脂用量(g)与上样液体积(m L)比例为1∶3~1∶4为宜,吸附时间10~11 h,70%乙醇以1.5 m L/min流速动态解吸10~11 h。结论:经D-101型大孔树脂纯化富集后,所得干浸膏中牛蒡苷和牛蒡苷元总量的质量分数提高了2倍以上。木脂素得到有效富集,确定的工艺条件稳定可行,重复性好,可实现工业化生产。该研究为牛蒡子木脂素相关药物的开发提供研究基础,为提高其临床用药价值提供技术支持。
OBJECTIVE: To optimize the technological conditions of macroporous resin enrichment and purification of lignans in Arctium lappa L. Methods: The specific adsorption capacity, specific desorption rate, adsorption rate and desorption rate of arctiin and arctigenin were used as indexes. The purification effect of five resins was compared by static adsorption-desorption test. By static adsorption-desorption kinetics curve , To determine the adsorption and desorption time; through single factor investigation to determine the desorbent concentration, sample concentration, flow rate and other related parameters. Results: The adsorption and desorption properties of lignan in Arctium lappa L. from D-101 resin were better. According to the test results and the actual production, the process conditions were as follows: the concentration of the sample was calculated to be 4.5-6.0 mg / m L, the ratio of the amount of resin (g) to the volume of the sample solution (m L) is 1: 3 to 1: 4, the adsorption time is 10 to 11 h, and 70% ethanol is desorbed dynamically at a flow rate of 1.5 m L / h Conclusion: After purification and enrichment of D-101 macroporous resin, the total content of arctiin and arctigenin in dried extract increased by more than 2 times. The lignans are effectively enriched, the process conditions are determined to be stable and feasible, and the repeatability is good, which can realize industrialized production. The research provided the research foundation for the development of Arctium lignan-related drugs and provided technical support for the improvement of its clinical value.