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目的探讨深低温停循环对大鼠海马线粒体的影响。方法 18只大鼠分为深低温停循环组、常温停循环组和常温不停循环组,每组6只,分别给予深低温停循环、常温停循环和常温不停循环处理。观察3组大鼠电镜下海马线粒体形态学、二维参数和三维参数变化。结果常温不停循环组大鼠海马线粒体呈长条形或椭圆形,基质均匀,大小正常,板状嵴及双层膜清晰;常温停循环组大鼠海马线粒体肿胀明显,部分呈空泡化,数量明显减少,板状嵴变形,双层膜破损,基质中出现絮状物;深低温停循环组海马线粒体轻度肿胀,数量减少,双层膜尚完整,板状嵴部分断裂,少数线粒体出现空泡化改变;深低温停循环组和常温停循环组大鼠海马线粒体直径[(116.22±7.23)、(154.26±6.41)μm]、周长[(433.6±15.2)、(594.7±17.0)μm]、灰度(131.2±12.4、133.4±11.8)、平均体积[(0.187±0.001)、(0.249±0.002)μm3]、数密度[(0.131±0.004)、(0.099±0.002)μm~2]和体积密度[(0.111±0.004)、(0.098±0.001)μm~(-3)]均大于常温不停循环组[(86.37±5.84)μm、(319.5±12.5)μm、108.7±8.6、(0.164±0.001)μm~3、(0.178±0.005)μm~2、(0.129±0.003)μm~(-3)](P<0.05),常温停循环组大鼠海马线粒体直径、周长、平均体积大于深低温停循环组,灰度、数密度和体积密度与深低温停循环组比较差异无统计学意义(P>0.05)。结论缺血缺氧能引起海马线粒体肿胀,数量减少,深低温能改善海马线粒体结构和数量的改变。
Objective To investigate the effects of deep hypothermic circulatory arrest on rat hippocampal mitochondria. Methods Eighteen rats were divided into deep hypothermic circulatory arrest group, normal circulatory arrest group and normal-temperature non-circulatory group, with 6 rats in each group. The rats were given deep hypothermic circulatory arrest, normal circulatory arrest, and normal-circulatory treatment. The changes of mitochondrial morphology, two-dimensional parameters and three-dimensional parameters of hippocampus in the three groups were observed under electron microscope. Results The mitochondria of rat hippocampus were elongated or oval in normal circulation group, the matrix was uniform, the size was normal, the plate ridge and bilayer membrane were clear. The mitochondria in hippocampus were obviously swollen, The number of mitochondria decreased, the number of mitochondria decreased, the number of mitochondria decreased slightly, the bilayer membrane was still intact, the plate-like crest was partly broken, and a few mitochondria appeared (116.22 ± 7.23), (154.26 ± 6.41) μm], and the circumference [(433.6 ± 15.2), (594.7 ± 17.0) μm] in the hippocampus of the hypothermic circulatory arrest group and the normal-temperature circulatory arrest group (0.221 ± 0.004), (0.099 ± 0.002) μm ~ 2], and the average volume [(0.187 ± 0.001), (0.249 ± 0.002) μm3] Volumetric density [(0.111 ± 0.004), (0.098 ± 0.001) μm ~ (-3)] were significantly higher than those in the normal circulation group [(86.37 ± 5.84) μm, (319.5 ± 12.5) μm, 0.001) μm ~ 3, (0.178 ± 0.005) μm ~ 2, (0.129 ± 0.003) μm -3] (P <0.05). The mean diameter of mitochondria, Low temperature Circular group, gradation, density and bulk density of the number of DHCA groups was not statistically significant (P> 0.05). Conclusion Hypoxia and hypoxia can cause mitochondria swelling in the hippocampus and decrease the number of the hippocampus. Deep hypothermia can improve the structure and quantity of the mitochondria in the hippocampus.