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目的:了解兰州市城区孕产妇叶酸知信行及相关卫生服务情况，为完善此项健康策略提供数据参考。方法:采用横断面研究方法，以年龄段分层随机选取2019年5月至2019年12月期间兰州市部分医疗机构孕检和住院分娩的孕产妇为研究对象，采用面对面问卷调查法获取叶酸知信行及接受相关卫生服务情况，通过多因素logistic回归分析其影响因素。结果:发放1060份问卷，共收回1060份，其中1046份有效问卷，有效率为98.70%。兰州市城区孕产妇的叶酸知识知晓率为79.06%（827/1046），其中问答叶酸可以预防胎儿神经管畸形的正确率为89.48%（936/1046）。叶酸服用率为89.29%（934/1046），其中孕前开始服用叶酸的孕产妇比例为48.82%（456/1046），规范服用叶酸孕产妇比例为40.04%（374/934）。卫生服务利用方面，仅19.91%（186/1046）的孕产妇领取免费叶酸。多因素logistic分析结果显示：文化教育程度为本科以下、家庭月收入2000~5999元（n OR=4.15，95% n CI=1.634~10.514）、认为补充叶酸没有必要（n OR=0.14，95%n CI=0.045~0.433）、认为目前叶酸政策/宣传没有作用（n OR=0.30，95% n CI=0.186~0.474）影响叶酸知识知晓率；孕次为1次（n OR=4.02，95%n CI=1.436~11.224）、有流产史（n OR=2.72，95% n CI=1.328~5.581）、不知晓叶酸知识（n OR=0.16，95% n CI=0.100~0.249）、认为补充叶酸没有必要（n OR=0.12，95% n CI=0.040~0.371）影响叶酸服用率。n 结论:兰州市城区孕产妇叶酸增补知识、行为有待提高，除个人、家庭因素外，现有相关卫生服务状况仍然存在覆盖面窄、宣传不到位、婚前教育缺乏针对性和叶酸发放渠道不畅通等诸多影响因素。提示应该从提高孕产妇健康意识和因地制宜地加强卫生服务有效性方面完善该地区叶酸增补策略。“,”Objective:To understand the knowledge, belief, practice and related health services of pregnant and lying-in women in Lanzhou City, provide data reference for improving this health strategy.Methods:A cross-sectional research method was used to randomly select pregnant and lying-in women who underwent pregnancy examinations and hospitalized deliveries in some medical institutions in Lanzhou City from May 2019 to December 2019 by age group as the research objects. Face-to-face questionnaire surveys were used to obtain folic acid knowledge, behavior and acceptance of related health services, and its influencing factors were analyzed by multivariate logistic regression.Results:Totally 1060 questionnaires were distributed, and 1060 questionnaires were recovered, of which 1046 were valid questionnaires, with an effective rate of 98.70%. The awareness rate of folic acid knowledge among pregnant and lying-in women in Lanzhou City was 79.06% (827/1046), and the correct rate of question and answer that folic acid could prevent fetal neural tube defects was 89.48% (936/1046). The rate of taking folic acid was 89.29% (934/1046). The proportion of pregnant women who started taking folic acid before pregnancy was 48.82% (456/1046), and the proportion of pregnant women taking folic acid was 40.04% (374/934). In terms of hygienic use, only 19.91% (186/1046) of pregnant women received free folic acid. The results of multivariate logistic analysis showed that education level is below undergraduate degree, family monthly income is 2000-5999 yuan (n OR=4.15, 95% n CI=1.634-10.514), folic acid supplementation is not necessary (n OR=0.14, 95% n CI=0.045-0.433), it is believed that the current folic acid policy/propaganda has no effect (n OR=0.30, 95% n CI=0.186-0.474) affected the awareness rate of folic acid knowledge; pregnancy times are 1 (n OR=4.02, 95% n CI=1.436-11.224), has miscarriage history (n OR=2.72, 95% n CI=1.328-5.581), no knowledge of folic acid (n OR=0.16, 95% n CI=0.100-0.249), folic acid supplementation is not necessary (n OR=0.12, 95% n CI=0.040-0.371) affected the rate of taking folic acid.n Conclusion:In Lanzhou, maternal folic acid supplementary knowledge, concepts, and behaviors need to be improved. Except for personal and family factors, the existing related health services still have narrow coverage, insufficient publicity, lack of targeted pre-marital education, and unavailable folic acid distribution channels. It is suggested that the folic acid supplementation strategy should be improved in terms of improving the health awareness of women of childbearing age and improving the effectiveness of health services according to local conditions.