论文部分内容阅读
目的观察和比较新孢子虫速殖子在人结肠癌细胞(HCT-8)、人宫颈癌细胞(Hela)和非洲绿猿肾细胞(Vero)中的生长情况。方法分别用HCT-8、Hela和Vero细胞(RPMI-1640培养基)连续培养新孢子虫速殖子9d,观察并计数速殖子数量,绘制生长曲线;4d取培养物作吖啶噔染色,用激光共聚焦显微镜观察速殖子。结果新孢子虫速殖子用HCT-8、Vero和Hela细胞培养均生长,尤以在HCT-8和Vero细胞中生长较快,第6d虫体数达到高峰,且在HCT-8中高峰期可持续2d,在Vero中生长高峰可持续1d。在Hela细胞中速殖子生长较慢,虫体数量一直处于较低水平。吖啶噔染色观察,HCT-8和Vero细胞中纳虫泡较大且速殖子数量较多,Hela细胞内纳虫泡较小且速殖子数量较少。结论新孢子虫速殖子在HCT-8细胞中生长较快,纳虫泡较大且速殖子数量较多。HCT-8细胞可以替代Vero细胞用于速殖子的体外培养。
Objective To observe and compare the growth of tachyzoites of Neospora in human colon cancer cells (HCT-8), human cervical cancer cells (Hela) and African green ape kidney cells (Vero). Methods Tachyzoites of Neospora were cultured continuously with HCT-8, Hela and Vero cells (RPMI-1640 medium) for 9 days, the number of tachyzoites was observed and counted, and the growth curve was drawn. The culture was stained with acridine for 4 days, Tachyzoites were observed with a laser confocal microscope. Results Tachyzoites of Neospora were grown in HCT-8, Vero and Hela cells, especially in HCT-8 and Vero cells. The number of parasites reached the peak on the 6th day and reached the peak in HCT-8 Sustainable 2d, the peak of growth in Vero sustainable 1d. Tachyzoites grew slowly in Hela cells, and the number of worms was always at a low level. Acridine 观 staining observed, HCT-8 and Vero cells in the larger size and the number of tachyzoites, Hela cells in the smaller and smaller number of tachyzoites. Conclusion The neosporosis tachyzoites grew faster in HCT-8 cells, with larger size and more tachyzoites. HCT-8 cells can replace Vero cells for tachyzoites in vitro.