hSHIP,a human SH2-containing inositol-5-phosphatase,acts as a negative regulator of proliferation and survival in hematopoietic cells.Therefore,hSHIP may play a crucial role in suppression of cervical cancer HeLa cells.In this study,pcDNA3.1-hSHIP-GFP plasmid was constructed and transfected into HeLa cells with Lipofectamine2000,stably transfected HeLa cells were established and their responses were investigated by Flow cytometry,MTT,tumorigenicity in nude mice,RT-PCR and ELISA assays.The results showed that the expression of hSHIP significantly induced S-phase arrest,cell growth inhibition,and down-regulation of Akt1/2 mRNA and p-Akt in HeLa cells.Our study supports an important role for hSHIP in suppression of cervical cancer HeLa cells,which may prove to be a novel therapeutic option for non-hematopoietic cancers.