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目的总结细胞损伤模型在化合物抗脑缺血活性筛选中的应用,旨在对药物的抗脑缺血研究提供新思路。方法参考国内外相关文献29篇,阐述急性脑缺血相关的基本理论以及目前常用的三种模拟体内病理进程的脑缺血体外细胞损伤模型的方法,并结合此三种细胞损伤模型,对细胞损伤模型如何用于新化合物的活性筛选进行了探讨。结果考察的三种体外模型涵盖了脑缺血时级联反应的三步重要进程,其中缺氧缺糖后复灌模型是最初损伤进程,而谷氨酸与H2O2模型是导致脑缺血损伤最严重的两步进程,模型符合脑缺血后病理损伤理论,具有一定的理论依据。结论可以利用三种模型初步判断化合物作用机制。
Objective To summarize the application of cell injury model in the screening of compounds against cerebral ischemic activity and to provide new ideas for anti-brain ischemia research of drugs. Methods With reference to 29 literatures at home and abroad, the basic theory of acute cerebral ischemia and the three commonly used methods of simulating the process of cerebral ischemia in vitro cell injury in vitro were described. Combined with these three cell injury models, How the damage model is used in the activity screening of new compounds is discussed. Results Three in vitro models examined the important three-step progression of the cascade in cerebral ischemia, in which the model of initial reperfusion after hypoxia-hypoglycemia was the initial lesion, and glutamate and H2O2 were the most common Serious two-step process, the model conforms to the theory of cerebral ischemic pathological damage, with a certain theoretical basis. Conclusion Three models can be used to determine the mechanism of action of compounds.