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目的采用热水浸提柚皮中柚皮苷,并使用大孔吸附树脂对其进一步富集,并考察其活性。方法以柚皮苷的吸附率和解吸率为考察指标,筛选不同大孔吸附树脂对柚皮苷的吸附性能和洗脱能力,并初步考察柚皮苷抗氧化作用和镇静作用。结果大孔树脂HPD450有较好的吸附效果,工艺条件:上样流速为2 BV·h-1,样液浓度为2.36 mg·mL-1;解吸最适工艺条件:洗脱剂乙醇浓度为50%,用量为4 BV,流速为3 BV·h-1。浓度为3.75 mg·mL-1的柚皮苷具有较好的抗氧化作用,以20 mg·kg-1给药时,小白鼠进入睡眠时间较短且行动比较缓慢。结论 HPD450可用于富集柚皮中的柚皮苷,得到的产物具有较好的镇静和安眠作用。
Objective To extract naringin from pomelo peel by hot water and further enrich it with macroporous adsorption resin and investigate its activity. Methods The adsorption and desorption rates of naringin were used as indexes to evaluate the adsorption and elucidation of naringin with different macroporous adsorption resins. The antioxidant and sedative effects of naringin were also investigated. Results The macroporous resin HPD450 had better adsorption performance. The process conditions were: the sample flow rate was 2 BV · h-1 and the sample concentration was 2.36 mg · mL-1. The optimal desorption conditions were as follows: the eluent ethanol concentration was 50 %, The amount of 4 BV, the flow rate of 3 BV · h-1. Naringin with a concentration of 3.75 mg · mL-1 had a good anti-oxidant effect. When administered at a dose of 20 mg · kg-1, the mice entered the sleep for a short time and were slow to act. Conclusion HPD450 can be used to enrich naringin in pomelo peel, and the obtained product has good sedative and hypnotic effects.