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目的 :研究人淋巴瘤细胞系凋亡蛋白酶 - 8(caspase- 8)对辐射诱导凋亡的作用。方法 :采用线性加速器 6 MV光量子 10 Gy照射 (剂量率4Gy/ m in) 8种人淋巴瘤细胞系。应用荧光显微镜和流式细胞仪定量分析白细胞分化抗原 95 (CD95 )刺激和辐射诱导的细胞凋亡 ,应用 Western印迹法
Objective: To investigate the effect of caspase-8 on radiation-induced apoptosis in human lymphoma cell line. METHODS: Eight human lymphoma cell lines irradiated with 6 Gy photons at a dose of 4 Gy / min were irradiated with a linear accelerator. Fluorescence microscopy and flow cytometry were used to quantitatively analyze the apoptosis induced by CD95 and radiation. Western blotting