目的用各种检测方法确定盐酸小檗碱片中人为掺入物的成分及掺入量。方法经TLC分离提取后,用核磁共振和质谱定性为异烟肼,再用异烟肼原料做TLC法、UV法、HPLC法对照实验。结果确证掺入物与异烟肼的色谱图和光谱图均一致。用UV法和HPLC法测定结果每片中异烟肼掺入量为14mg左右。结论测定结果与样品实际测定情况完全吻合。 Objective To determine the composition and incorporation of artificial admixture in various kinds of test methods. Methods After isolation and separation by TLC, it was identified as isoniazid by nuclear magnetic resonance and mass spectrometry. Then the isoniazid was used as raw material for TLC, UV and HPLC. The results confirm that both the chromatogram and the spectrogram of the isoside and isoniazid are consistent. UV and HPLC determination of the results of each piece of isoniazid incorporation of about 14mg. Conclusion The results of the determination and the actual determination of the sample exactly match.