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利用HPLC法比较分析旱生与湿生环境下蒲公英全草及各器官绿原酸含量差异,探讨影响植物生长和次生物质代谢的主要因子--生境对蒲公英绿原酸含量的影响,为进一步利用蒲公英和调节绿原酸代谢提供依据。色谱条件为Inertsil ODS-SP色谱柱(150mm×4.6,5μm);流动相为甲醇-0.4%磷酸水溶液(15:85);检测波长326nm;柱温35℃;流速1m L/min;进样量10μL。检测结果表明,旱生蒲公英全草及各器官的绿原酸含量明显高于湿生蒲公英,其全草的绿原酸含量相差近8倍。不同器官中旱生蒲公英叶的绿原酸含量最高,湿生蒲公英根的绿原酸含量最高。
HPLC method was used to analyze the differences of chlorogenic acid content between whole plant and organs under drought and wet conditions, and to explore the effect of habitat on the chlorogenic acid content of dandelion, which is the main factor affecting plant growth and secondary metabolites. The use of dandelion and regulate chlorogenic acid metabolism provide the basis. The chromatographic conditions were Inertsil ODS-SP column (150 mm × 4.6, 5 μm); the mobile phase was methanol-0.4% phosphoric acid solution (15:85); the detection wavelength was 326 nm; the column temperature was 35 ℃; the flow rate was 1 m L / 10μL. The results showed that the content of chlorogenic acid in whole plant and organs of dry dandelion was significantly higher than that of wet dandelion, and the content of chlorogenic acid in whole plant differed by nearly 8 times. In different organs, the content of chlorogenic acid in the leaf of xerophytic dandelion was the highest, and the content of chlorogenic acid in the root of wet dandelion was the highest.