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刪除肝癌2(DLC2),一种经常发现在原发性肝癌过低表达的肿瘤抑制基因,编码一种含有不育-α-基序多域蛋白质(DLC2-SAM).以前SAM域蛋白(DLC2-SAM)核磁共振结构显示此蛋白是由独特的四螺旋束组成,与其它已知SAM域结构截然不同.在该研究中,作者运用了15N-1H残留偶极偶合(RDC)作为附加约束连同NOE和TA-LOS数据进一步优化了DLC2-SAM的结构.由此所得的结构与没有15N-1H残留偶极偶合约束比较显示改善了结构的质量并且有较低的Q值.螺旋的取向,尤其是螺旋4,被残留偶极偶合数据所验证.RDC-优化的人类DLC2-SAM的结构与小鼠的DLC2-SAM很相像.DLC家庭独特的SAM域结构表明该域可能还具有没被发现的新功能.
Deletion of Liver Cancer 2 (DLC2), a tumor suppressor gene frequently found to be under-expressed in primary hepatocarcinomas, encodes a protein that contains an infertility-α-motif multidomain protein (DLC2-SAM) -SAM) NMR structure shows that this protein is composed of a unique quad helix bundle and is distinctly different from other known SAM domain structures.In this study, the authors used 15N-1H Residue Dipolar Coupling (RDC) as an additional constraint along with NOE and TA-LOS data further optimized the structure of DLC2-SAM The structure thus obtained shows improved structural quality with lower Q values compared to the 15N-1H residual dipole coupling constraint.The orientation of the helix, in particular Is helix 4 and is validated by residual dipole coupling data.The structure of RDC-optimized human DLC2-SAM resembles that of mouse DLC2-SAM.The unique SAM domain structure of the DLC family suggests that this domain may also have undetected new function.