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目的比较淡色库蚊抗吡丙醚种群和敏感种群羧酸酯酶生化特征的差异,初步探讨其生化抗性机制。方法参照van Aspern(1962)的方法测定羧酸酯酶活性。结果淡色库蚊抗性品系和敏感品系羧酸酯酶水解活性随底物浓度(α-乙酸萘酯或β-乙酸萘酯)的增加而升高;在一定浓度范围内,抗性品系对这两种底物的水解活性均高于敏感品系。β-乙酸萘酯为底物时,抗性品系羧酸酯酶的米氏常数(K_m)和最大反应速度(V_(max))分别为27.20×10~(-5)mol/L和115.00×10~(-5)A/(mg·pro·min),敏感品系K_m和V_(max)分别为104.00×10~(-5)mol/L和207.00×10~(-5)A/(mg·pro·min),两者差异有统计学意义(t_b=2.740,t_c=3.160,P<0.05)。α-乙酸萘酯为底物时,抗性品系羧酸酯酶K_m为131.00×10~(-5)mol/L,与敏感品系K_m(75.20×10~(-5)mol/L)比较差异无统计学意义,而V_(max)则差异有统计学意义。胆碱酯酶抑制剂测定结果表明,抗性品系羧酸酯酶对敌敌畏和残杀威的敏感性高于敏感品系。结论羧酸酯酶可能与淡色库蚊对吡丙醚的抗性有关。
Objective To compare the biochemical characteristics of carboxylic acid esterase between Culex pipiens quinquefasciatus and susceptible populations and to explore the biochemical resistance mechanism. Methods Carboxylesterase activity was measured by the method of van Aspern (1962). Results The carboxylesterase activity of resistant and susceptible strains of Culex pipiens pallens tended to increase with increasing substrate concentration (α-naphthyl acetate or β-naphthyl acetate). In a certain concentration range, The hydrolytic activities of the two substrates were higher than those of the sensitive strains. The Km and Vmax of resistant carboxylesterase were 27.20 × 10 -5 mol / L and 115.00 × 10 -5 A / mg · pro · min and the sensitive strains K_m and V_max were 104.00 × 10 -5 mol / L and 207.00 × 10 -5 A / mg, respectively · Pro · min), the difference was statistically significant (t_b = 2.740, t_c = 3.160, P <0.05). The results showed that the carboxylesterase K_m of the resistant strain was 131.00 × 10 -5 mol / L, which was significantly lower than that of the susceptible strain K_m (75.20 × 10 -5 mol / L) No statistical significance, while the V_ (max) the difference was statistically significant. Choline esterase inhibitor assay results showed that the resistant strains of carboxylesterase were more sensitive to dichlorvos and propoxur than sensitive strains. Conclusion Carboxylesterase may be related to the resistance of Culex pipiens to pyriproxyfen.