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本文研究orexinA(OXA)和orexin 1型受体(OX1R)在新生大鼠与成年大鼠延髓的分布及比较。新生(1～5d)和成年(6～8周)SD大鼠,取其延髓,采用免疫组织化学方法和图像分析技术,观察OXA和OX1R在新生与成年SD大鼠延髓的分布。结果显示,OXA免疫反应阳性纤维和OX1R免疫反应阳性细胞在新生和成年SD大鼠延髓内有广泛分布,主要分布在延髓腹外侧区(ventrolateralmedulla,VLM)和舌下神经核(hypoglossalnucleus,XII)。在这两个区域,新生大鼠组的OX1R免疫反应阳性细胞的相对光密度值均低于成年大鼠组(P<0.001)。结果表明随着大鼠发育成熟,OX1R表达水平增加,可能与其生理功能完善有关。OXA免疫反应纤维和OX1R免疫反应阳性细胞在延髓的VLM和XII的表达提示可能与心血管和呼吸等活动的调节有关。
This study was to investigate the distribution and comparison of orexinA (OXA) and orexin type 1 receptor (OX1R) in the medulla oblongata of neonatal and adult rats. (1 ~ 5d) and adult (6 ~ 8 weeks) SD rats. The medulla oblongata was taken for immunohistochemistry and image analysis to observe the distribution of OXA and OX1R in the medulla oblongata of neonatal and adult SD rats. The results showed that OXA immunoreactive fibers and OX1R immunoreactive cells were widely distributed in the medulla oblongata of neonatal and adult SD rats, mainly in ventrolateral medulla (VLM) and hypoglossal nucleus (XII). In both regions, the relative optical density values of OX1R immunoreactive cells in neonatal rats were lower than those in adult rats (P <0.001). The results showed that with the development of rats, OX1R expression increased, which may be related to its physiological function. OXA immunoreactive fibers and OX1R immunoreactive cells in the medulla oblongata VLM and XII expression may be related to the regulation of cardiovascular and respiratory and other activities.