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目的为防治非酒精脂肪性肝炎(NASH)的研究提供理想的动物模型。方法将C57BL/6雄性小鼠和SD大鼠分别随机分成2组,分别喂养蛋氨酸-胆碱充足(MCS)饲料(正常组)和蛋氨酸-胆碱缺乏(MCD)饲料(模型组)。分别在各组中饲养3、5、8周后,取动物血清和肝脏。测定血清的谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)和胆固醇(TC)的含量;肝脏切片后经HE染色,观察肝脏的病理形态学变化并对其病变程度进行评分。结果饲喂MCD饲料5周后,SD大鼠血清AST含量无显著变化(P>0.05),但是C57BL/6小鼠AST水平显著升高(P<0.05)。同时,SD大鼠和C57BL/6小鼠模型的血清ALT水平均显著升高(P<0.05),此外,SD大鼠和C57BL/6小鼠血清中TG和TC的水平均显著下降(P<0.05),肝组织的病理评分均出现显著升高(P<0.05)。但是C57BL/6小鼠血清AST、ALT的变化幅度及肝组织病理评分明显高于SD大鼠。结论 C57BL/6小鼠NASH模型优于SD大鼠NASH模型。C57BL/6小鼠NASH模型相对SD大鼠,更适合NASH的分子机制和防治药物的评价。
Objective To provide ideal animal models for the prevention and treatment of non-alcoholic steatohepatitis (NASH). Methods C57BL / 6 male and SD rats were randomly divided into two groups: MCF - fed group (normal group) and methionine - choline deficiency (MCD) group (model group). After being housed for 3,5,8 weeks in each group, animal serum and liver were taken. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG) and cholesterol (TC) were measured. The liver sections were stained with HE to observe the pathological changes of the liver, score. Results Serum AST did not change significantly in Mice fed with MCD for 5 weeks (P> 0.05), but AST in C57BL / 6 mice was significantly increased (P <0.05). At the same time, the levels of serum ALT in SD rats and C57BL / 6 mice were significantly increased (P <0.05). In addition, the levels of TG and TC in serum of SD rats and C57BL / 6 mice were significantly decreased (P < 0.05). The pathological scores of liver tissue were significantly increased (P <0.05). However, the changes of AST and ALT in C57BL / 6 mice were significantly higher than those in SD rats. Conclusion The NASH model of C57BL / 6 mice is better than that of NASH model of SD rats. Compared with SD rats, the C57BL / 6 mouse NASH model is more suitable for the molecular mechanism of NASH and evaluation of drug control.