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以中豆-31为材料,采用水培方法,研究了不同浓度的水杨酸(SA)对4℃低温胁迫下大豆幼苗生长的影响。结果表明,低温胁迫下,大豆幼苗叶绿素和可溶性蛋白含量、根系活力显著降低,可溶性糖、游离脯氨酸(Pro)、丙二醛(MDA)含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)活性显著提高(P<0.01);外施SA显著提高了低温胁迫下幼苗叶片的叶绿素、可溶性蛋白、可溶性糖和Pro含量,SOD、POD活性和根系活力,显著降低了膜脂过氧化产物MDA含量(P<0.01)。由此可见,外施SA可以通过提高大豆幼苗可溶性蛋白、可溶性糖、Pro含量以及SOD、POD活性来维持细胞膜的稳定性,降低膜脂过氧化伤害程度,从而缓解低温胁迫对幼苗生长的抑制,并以1.5 mmol.L-1外源SA效果最好。
Using medium-soybean-31 as material, the effects of different concentrations of salicylic acid (SA) on the growth of soybean seedlings under low temperature stress of 4 ℃ were studied by hydroponics. The results showed that under cold stress, the content of chlorophyll, soluble protein and root activity of soybean seedling decreased significantly, and the content of soluble sugar, free proline (Pro), malondialdehyde (MDA) and superoxide dismutase (SOD) (P <0.01). Exogenous SA significantly increased the content of chlorophyll, soluble protein, soluble sugar and Pro, the activity of SOD and POD and the root activity of seedling leaves under low temperature stress, and significantly decreased the membrane lipid Peroxidation product MDA content (P <0.01). Thus, SA can improve the stability of the cell membrane and reduce the damage of membrane lipid peroxidation by improving the soluble protein, soluble sugar, Pro content and the activities of SOD and POD in soybean seedlings, so as to alleviate the inhibition of seedling growth under low temperature stress, And 1.5 mmol.L-1 exogenous SA best.