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作者用免疫组化和胶体金免疫电镜方法,观察乙型肝炎x抗原(HBxAg)在肝癌组织(35例)及癌旁肝组织(29例)内的分布及亚细胞定位。结果显示,HBxAg在癌组织及癌旁肝组织内的检出率分别为60.0％和82.8％,阳性物质主要位于细胞浆内。电镜观察,HBxAg主要定位于粗面内质网和胞浆基质中,少数位于核内,并可见游离核糖体及核孔附近阳性,推测HBxAg在内质网及游离核糖体上合成,并可经核孔进入核内。
The authors used immunohistochemistry and colloidal gold immunoelectron microscopy to observe the distribution and subcellular localization of hepatitis B x antigen (HBxAg) in hepatocellular carcinoma (35 cases) and adjacent liver tissue (29 cases). The results showed that the detection rates of HBxAg in cancer tissues and adjacent liver tissues were 60.0% and 82.8%, respectively. Positive substances were mainly located in the cytoplasm. Electron microscopy showed that HBxAg was mainly localized in the rough endoplasmic reticulum and cytoplasmic matrices, and a few were located in the nucleus. Positive ribosome and nuclear pores were seen nearby. HBxAg was presumably synthesized on the endoplasmic reticulum and free ribosomes, and Nuclear holes enter the nucleus.