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目的对疑似流感疫情标本进行核酸检测、明确感染因子,为邵阳市流感的防控提供科学依据。方法采用RT-PCR法及Real-Time RT-PCR法对可疑咽拭子标本进行流感病毒核酸分型检测。结果 958份可疑咽拭子标本中有483份流感病毒核酸阳性,阳性率50.42%,其中季节性甲型(甲1亚型/甲3亚型)流感核酸阳性107份,占11.17%;乙型流感核酸阳性13份,占1.36%;甲型H1N1核酸阳性363份,占37.89%.7~24岁年龄段H1N1检测阳性率为73.83%。60岁以上年龄段阳性率仅为1.38%。结论 2009年邵阳市检出的流感流行病毒株以甲型H1N1流感病毒为主。Real-Time RT-PCR技术是流感疫情临床病例诊断可靠的快速诊断方法。
Objective To detect the nucleic acid of the suspected influenza outbreak and clarify the infection factors to provide a scientific basis for the prevention and control of influenza in Shaoyang City. Methods RT-PCR and Real-Time RT-PCR were used to detect the influenza virus in suspicious throat swab samples. Results A total of 483 influenza virus nucleic acid positive samples were obtained from 958 suspicious throat swab specimens, with a positive rate of 50.42%. Of them, 107 were positive for seasonal influenza A (subtype A / subtype A3) influenza virus, accounting for 11.17% Influenza nucleic acid positive 13, accounting for 1.36%; A H1N1 nucleic acid positive 363, accounting for 37.89% .7 to 24 age group H1N1 positive rate was 73.83%. The positive rate of age over 60 years old was only 1.38%. Conclusion In 2009, the influenza A (H1N1) virus was detected in Shaoyang City. Real-Time RT-PCR is a reliable and rapid diagnostic method for clinical cases of influenza.