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宫颈癌病因复杂，多数研究者认为是多因素综合作用的结果。本文通过病例－对照研究，应用分子及血清流行病学方法，探讨引起宫颈癌的危险因素。应用ＨＰＶ６／１１、ＨＰＶ１６／１８探针，通过斑点分子杂交技术，检测宫颈癌和对照宫颈组织ＤＮＡ中的感染，结果病例组和对照组ＨＰＶ６／１１阳性率分别为２４．６％、２７．５％，差别无显著性，ＨＰｙ１６／１８阳性率分别为４５．６％、９．８％，差别有显著性；应用ＰＣＲ技术检测两组宫颈组织ＤＮＡ中ＨＳＶ－２感染，结果病例组和对照组阳性率分别为２８．１％、７．８％，Ｌｏｇｉｓｔｉｃ回归平衡年龄因素后差别无显著性；应用ＥＬＩＳＡ方法检测血清中ＨＣＭＶ抗体阳性率，结果病例组和对照组ＩｇＭ阳性率分别为１２．３％、５．９％，差别无显著性。用多因素Ｌｏｇｉｓｔｉｃ回归平衡各因素间的作用，最后分析结果表明，文化程度、产次、ＨＰＶ１０／１８感染与宫颈癌的发生有关，其ＯＲ值分别为０．５７、１．８５、３１．９６。
The etiology of cervical cancer is complex, most researchers think is the result of multi-factor combination. In this paper, case-control studies, molecular and serum epidemiological methods to explore the risk of cervical cancer risk factors. HPV6 / 11 and HPV16 / 18 probes were used to detect HPV DNA in cervical cancer and control cervical tissue by dot blot hybridization. The positive rates of HPV6 / 11 in case group and control group were 24.6% and 27.5 %, The difference was not significant, the positive rates of HPy16 / 18 were 45.6% and 9.8% respectively, the difference was significant. PCR was used to detect the HSV-2 infection in the cervical tissue DNA of both groups. Results The cases and control groups The positive rates were 28.1% and 7.8%, respectively. There was no significant difference between the age-adjusted logistic regression analysis. The positive rate of serum HCMV antibody was detected by ELISA. The positive rates of IgM in the cases and controls were 12.3% %, 5.9%, the difference was not significant. Multivariate Logistic regression was used to balance the effects of various factors. The final analysis showed that the educational level, parity, HPV10 / 18 infection were associated with the occurrence of cervical cancer, the OR values were 0.57, 1.85, 31.96 .