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目的:研究纵条纹炭角菌子实体的化学成分。方法:采用硅胶柱层析、Sephadex LH-20柱色谱及半制备型HPLC等色谱方法进行分离纯化,根据波谱数据鉴定化合物的结构。采用MTT法对化合物1~3、7进行体外肿瘤细胞毒活性测试。结果:从纵条纹炭角菌子实体的95%乙醇提取物中分离得到10个化合物,分别鉴定为:细胞松弛素D(1)、19,20-环氧细胞松弛素Q(2)、细胞松弛素Q(3)、19,20-环氧细胞松弛素D(4)、19,20-环氧细胞松弛素C(5)、反式对羟基肉桂酸乙酯(6)、吲哚-3-甲醛(7)、次黄嘌呤核苷(8)、尿嘧啶(9)、尿嘧啶核苷(10)。体外肿瘤细胞毒活性实验结果显示化合物7(40μmol/L)对HCT116、B16、U937肿瘤细胞的相对抑制率分别为57.12%、53.39%、57.85%。结论:所有化合物均首次从该菌中分离得到,其中,化合物7具有中等的肿瘤细胞毒活性。
Objective: To study the chemical constituents of Verticillasia arborescens. Methods: The compounds were separated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. The structures of the compounds were identified by spectral data. Compounds 1 ~ 3,7 were tested for cytotoxicity in vitro by MTT assay. Results: Ten compounds were isolated from the 95% ethanol extract of C. tabaci, identified as cytochalasin D (1), 19,20-epidermal relaxin Q (2), cytochalasis (3), 19,20-Epothilone D (4), 19,20-Epicytochalasin C (5), Ethyl 6-Hydroxycinnamate - Formaldehyde (7), Inosine (8), Uracil (9), Uridine (10). In vitro cytotoxicity of tumor cells showed that the relative inhibitory rates of compound 7 (40μmol / L) on HCT116, B16 and U937 tumor cells were 57.12%, 53.39% and 57.85%, respectively. Conclusion: All the compounds were isolated from this strain for the first time. Among them, compound 7 possessed moderate tumor cytotoxic activity.