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飞秒激光双光子显微成像技术在神经科学研究中发挥着重要作用.实验中采用自行改进的双光子成像技术探测大鼠皮层脑片神经元钙活动,记录到自发以及电刺激、药物刺激诱导的钙活动,同时记录到飞秒激光诱发的神经钙活动.结果表明,神经元钙升高的幅度与其对应电活动强度呈线性关系;谷氨酸能够诱导神经元产生大幅度的钙升高,但当多次刺激时其钙响应的幅度降低;通过同时记录多个神经元的自发钙信号,可以分析判断这些细胞属于不同的微回路;飞秒激光能诱导细胞的局部钙升高和整体钙升高.飞秒激光神经活动测量和控制方法具有非接触、无损伤、可精确重复的优点,为进一步认识神经生物现象提供了行之有效的实验手段.
Femtosecond laser two-photon microscopy imaging technology plays an important role in neuroscience research.In this experiment, using self-modified two-photon imaging detection of cortical neuronal calcium activity in rats, recorded spontaneous and electrical stimulation, drug-induced induction , And recorded the femtosecond laser-induced activity of calcium.The results showed that the amplitude of neuronal calcium increased linearly with its corresponding electrical activity intensity.Glutamate could induce the neurons to produce a significant increase of calcium, However, the magnitude of calcium response decreased when stimulated for many times. By recording the spontaneous calcium signals from multiple neurons at the same time, these cells could be analyzed to determine that the cells belong to different microcircuits. Femtosecond laser can induce local calcium elevation and global calcium And the femtosecond laser nerve activity measurement and control method has the advantages of non-contact, no damage and accurate repetition, and provides an effective experimental method for further understanding neurobiology.