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为探讨输精管结扎对睾丸曲细精管生精细胞凋亡程度的影响，应用流式细胞仪和脱氧核糖核酸末端转移酶介导的ｄＵＴＰ缺口末端标记（ＴＵＮＥＬ）技术，检测大鼠输精管结扎和假手术后２～１２周睾丸生精细胞的凋亡情况。结果：流式细胞仪检测发现结扎组的亚单倍体峰增高，亚单倍体细胞百分率升高；ＴＵＮＥＬ法则显示结扎后总的生精细胞凋亡数较假手术后明显增加（Ｐ＜０．００１）。结扎后不同时间组与同期假手术组比较，２周、６周、１０周组精原细胞和初级精母细胞凋亡数前者均高于后者（Ｐ均＜０．０５），１２周组精原细胞凋亡数前者高于后者（Ｐ＜０．０５）。结果表明输精管结扎会导致生精细胞凋亡增加。
To investigate the effect of vasectomy on the apoptosis of seminiferous tubules in rats, flow cytometry and DNA nick end-transferase-mediated dUTP nick end labeling (TUNEL) were used to detect vasectomy and artificial hand Apoptosis of spermatogenic cells in testis 2 ~ 12 weeks after operation. Results: The results of flow cytometry showed that the sub-haploid peak in the ligation group was increased and the percentage of sub-haploid cells was increased. The TUNEL method showed that the total number of apoptotic spermatogenic cells increased significantly after ligation (P <0.05) .001). The number of apoptotic spermatogonia and primary spermatocyte in the two groups were significantly higher than those in the sham-operation group (P <0.05) at 12 weeks, 2 weeks, 6 weeks and 10 weeks after ligation, The former spermatogonial apoptosis was higher than the latter (P <0.05). The results show that vasectomy lead to increased apoptosis of spermatogenic cells.