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目的了解丽水市中心医院临床分离鲍曼不动杆菌的耐药特征及通过脉冲场凝胶电泳技术了解菌株的分子流行病学特征。方法收集丽水市中心医院分离的139株鲍曼不动杆菌,采用K-B法检测菌株对抗生素的耐药性;随机选取30株多重耐药鲍曼不动杆菌进行PFGE及聚类分析。结果 139株鲍曼不动杆菌感染患者中,>60岁的比例较高,占66.19%;鲍曼不动杆菌的检出率在痰液标本中最高(90.65%),主要分布于ICU。该菌对多粘菌素B均敏感,其余抗菌药物的耐药率均在66%以上。聚类分析表明,30株鲍曼不动杆菌共分为4个PFGE群,依次命名为A~D,其中A群为主要流行克隆,共23株,占76.67%。结论鲍曼不动杆菌已成为丽水市中心医院感染的重要病原菌,在多个科室播散,主要分布于ICU病区。克隆菌株的播散是造成本院鲍曼不动杆菌分离率、耐药率上升的主要原因。
OBJECTIVE To understand the drug resistance of Acinetobacter baumannii isolates from Lishui Central Hospital and to understand the molecular epidemiological characteristics of the strains by pulsed-field gel electrophoresis. Methods 139 strains of Acinetobacter baumannii isolated from the Central Hospital of Lishui City were collected. The antibiotic resistance of the strains was tested by K-B method. Thirty strains of multi-drug resistant Acinetobacter baumannii were selected randomly for PFGE and cluster analysis. Results Of the 139 Acinetobacter baumannii infections, the prevalence rate was> 60 years old, accounting for 66.19%. The detection rate of Acinetobacter baumannii was the highest (90.65%) in sputum samples and mainly distributed in ICU. The bacteria are sensitive to polymyxin B, the remaining antibiotic resistance rates were 66% or more. Cluster analysis showed that 30 strains of Acinetobacter baumannii were divided into four PFGE groups, named as A ~ D in turn. Among them, group A was the major epidemic clone, accounting for 76.67%. Conclusion Acinetobacter baumannii has become an important pathogen of central hospital infection in Lishui City and spreads in many departments and is mainly distributed in the ICU ward. The dissemination of clonal strains is the main cause of Acinetobacter baumannii isolation rate and resistance rate in our hospital.