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使用不同稀释度的蛇毒毒素，等体积注射于大鼠背部皮下、皮内和小鼠背部皮下，１８～２４小时剥皮观察比较动物皮下出血程度，经统计学处理，小鼠背部皮下注射对检查出血毒最敏感。选用小鼠背部皮下注射０．２ｕ蛇毒酶成品、半成品，１８～２４小时剥皮观察皮下瘀血或瘀斑，发现４２批成品中８８．１％未见皮下瘀血，２４批半成品７５％未见皮下瘀血。说明选用小鼠背部皮下注射０．２ｕ蛇毒酶来限量检查出血毒的方法是可取的。
Using different dilutions of the snake venom toxin, the same volume injection in the back of the rat subcutaneous, intradermal and subcutaneous back of mice, 18 to 24 hours of skin peel observed and compared the degree of animal subcutaneous bleeding after statistical analysis, the back of the mouse subcutaneous injection to check the bleeding Poison is the most sensitive. Subcutaneous injection of 0.2u snake venom enzyme finished product and semi-finished product was performed on the back of the mice, and subcutaneous bleeding or ecchymosis was observed after peeling for 18-24 hours. 88.1% of the 42 batches were found no subcutaneous bleeding and 75% of 24 batches of semi-finished products were not seen Subcutaneous bleeding. Description of mouse subcutaneous injection of 0.2u venom enzyme to limit the detection of bleeding poison method is desirable.