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目的 :检测喉癌下咽癌组织中转化生长因子 β1(TGF β1)mRNA的表达 ,探讨其在喉癌、下咽癌发生发展中的可能作用。方法 :取 2 0例喉癌、下咽癌患者术后新鲜组织标本 ,用半定量的逆转录 聚合酶链反应 (RT PCR)方法检测TGF β1的mRNA表达。结果 :检测 2 0例标本 ,其中 17例TGF β1表达阳性 ,且Ⅲ～Ⅳ期表达较Ⅰ～Ⅱ期强。结论 :TGF β1在喉癌、下咽癌中的表达可能是导致喉癌、下咽癌患者免疫功能低下 ,促进肿瘤生长的原因之一
Objective: To detect the expression of transforming growth factor β1 (TGFβ1) mRNA in laryngeal carcinoma hypopharyngeal carcinoma and to explore its possible role in the development of laryngeal carcinoma and hypopharyngeal carcinoma. Methods: Tissue specimens from 20 laryngeal and hypopharyngeal cancer patients were collected. The mRNA expression of TGF-β1 was detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: Twenty cases of specimens were detected. Among them, 17 cases had positive expression of TGFβ1 and the expression in stage Ⅲ ~ Ⅳ was stronger than that in stage Ⅰ ~ Ⅱ. CONCLUSION: The expression of TGF-β1 in laryngeal and hypopharyngeal carcinomas may be one of the reasons for the poor immunocompromising and tumor growth in laryngeal and hypopharyngeal cancer patients