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轮状病毒是引起人类和动物急性腹泻的传染病毒,其外壳蛋白VP7决定血清型G型并在诱导产生中和抗体中具有重要的作用,NSP4蛋白作为轮状病毒致泻蛋白,在疫苗研究中亦备受瞩目。本文应用最大似然法分析中国区G1～G3型VP7、NSP4蛋白编码序列。其中VP7序列未发现正选择位点,NSP4中共发现3个正选择位点,这些经历正选择作用的位点位于不同的序列功能区内,暗示这些位点可能对相关的功能贡献决定性作用。本研究的结果为轮状病毒的免疫研究提供参考。
Rotavirus is an infectious virus that causes acute diarrhea in humans and animals and its coat protein VP7 determines serotype G and plays an important role in inducing the production of neutralizing antibodies. The NSP4 protein, as a rotavirus diarrhea protein, Also attracted much attention. In this paper, the maximum likelihood method was used to analyze the coding sequences of VP7 and NSP4 in China from G1 to G3. There were no positive selection sites in VP7 sequence and three positive selection sites in NSP4. These positive selection sites were located in different functional domains, suggesting that these sites may play a decisive role in the related functions. The results of this study provide a reference for the immunological research of rotavirus.