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目的:拟建立用高效液相色谱法测定复方丙谷胺片中丙谷胺含量的方法。方法:采用Diamonsil C18色谱柱(250 mm×4.6 mm,5μm),以2%醋酸铵-甲醇(40∶60)为流动相,检测波长为225 nm,外标法计算含量。结果:丙谷胺在2.60~208.10μg与峰面积有良好的线性关系,r=0.999 9,平均加样回收率为99.8%,RSD为0.5%(n=6)。结论:HPLC法测定复方丙谷胺片中丙谷胺含量准确度高,操作简单,重复性好。
Objective: To establish a method for the determination of proglumide in compound promethazine tablets by high performance liquid chromatography. Methods: Diamonsil C18 column (250 mm × 4.6 mm, 5 μm) with 2% ammonium acetate-methanol (40:60) was used as the mobile phase. The detection wavelength was 225 nm. RESULTS: Prothymine had a good linear relationship with the peak area at 2.60 ~ 208.10μg, r = 0.999 9, the average recovery was 99.8% and the RSD was 0.5% (n = 6). Conclusion: The HPLC method for the determination of plasma proglummine tablets with high accuracy, simple operation, good reproducibility.