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为探讨豚鼠胆汁中胃动素（ＭＴＬ）、血管活性肠肽（ＶＩＰ）和生长抑素（ＳＳ）的来源、释放调节及其在胆囊结石形成中的作用，采用放射免疫分析（ＲＩＡ）方法分别测定了正常对照组、致石组及恢复组豚鼠胆汁、胆囊壁组织及门静脉血中ＭＴＬ、ＶＩＰ和ＳＳ的含量以及胆汁理化性质变化。结果：正常对照组豚鼠胆汁中ＭＴＬ、ＶＩＰ及ＳＳ含量均高于门静脉血，而胆囊组织中则含有较高水平的胃肠激素。与正常对照组比较，致石组胆汁中ＭＴＬ降低，ＶＩＰ和ＳＳ增高（Ｐ＜０．０１），胆汁性质发生致石性改变；恢复组胆汁的胃肠激素及理化性质均恢复正常（Ｐ＜０．０５）。本实验结果提示：豚鼠胆汁的ＭＴＬ、ＶＩＰ及ＳＳ等胃肠激素可能主要来自胆囊壁组织；食物成分影响胆汁中的胃肠激素含量；致石试验时胆汁中ＭＴＬ、ＶＩＰ及ＳＳ的变化促使胆汁理化性质的致石改变及胆石的生成。
To investigate the origin and release regulation of motilin (MTL), vasoactive intestinal peptide (VIP) and somatostatin (SS) in guinea pig bile and its role in gallstone formation, radioimmunoassay (RIA) The contents of MTL, VIP and SS in bile, gallbladder wall tissue and portal vein blood in guinea pigs and the changes of bile physicochemical properties were measured in normal control group, Zhishi group and recovery group. Results: The content of MTL, VIP and SS in guinea pigs in normal control group were higher than those in portal vein, while the levels of gut hormone in gallbladder tissues were higher. Compared with the normal control group, MTL decreased, VIP and SS increased (P <0.01), and bile properties changed into stone in the stone group. The gut hormone and physicochemical properties of the bile in the recovery group returned to normal (P < 0.05). The results of this study suggest that the gastrointestinal hormones such as MTL, VIP and SS in guinea pig bile may mainly come from the gallbladder wall tissue. The food composition affects the content of gut hormones in bile; the changes of MTL, VIP and SS in bile during the stone test lead to the change of bile Physical and chemical properties caused by changes in stone and gallstone formation.