论文部分内容阅读
目的 探讨PRR11蛋白在胃癌(gastric cancer,GC)中的表达,分析其表达异常与GC进展和预后的关系.方法 应用免疫组织化学(immunohistochemistry,IHC)法检测PRR11在436例GC组织中的表达,并用免疫印迹(Western blot)比较GC和正常胃黏膜组织中PRR11的表达,统计学分析其与临床病理参数之间的关系.结果 Western blot定量分析中PRR11在GC组织中的表达显著高于正常胃黏膜组织;同时IHC显示PRR11在GC中呈特异性表达,其阳性定位于胞浆和胞膜,GC组织阳性表达率为41.8％(182/436),而在正常胃黏膜组织中不表达或微弱表达.PRR11的过表达与GC的分化程度(P＜0.01)、浸润深度(P=0.03)、TNM分期(P＜0.01)、淋巴结转移(P＜0.01)和远处转移(P=0.05)密切相关.结论 PRR11在GC中过表达,过表达的PRR11与GC的发生、进展以及预后密切相关,PRR11可作为判断GC患者预后的重要指标.“,”AIM To detect the expression of PRR11 in gastric cancer (GC) and to analyze its relationship with GC progression and prognosis.METHODS Expression of PRR11 in 436 GC samples was detected by immunohistochemistry (IHC),and Western blot was used to compare PRR11 expression between GC samples and normal gastric mucosal samples.The correlation of PRR11 expression with clinicopathological parameters was analyzed.RESULTS Western blot analysis showed that the expression of PRR11 in GC samples was obviously higher than that in normal gastric mucosal samples.IHC results showed that PRR11 was specifically expressed in GC and located in the cytoplasm and membrane;PRR11 was overexpressed in 182 (41.8％) of GC samples,but none or weak expression was detected in the normal gastric mucosa.PRR11 overexpression was correlated significantly with clinicopathologic features such as tumor differentiation (P ＜ 0.01),tumor invasion (P =0.03),TNM stage (P ＜ 0.01),lymph node metastasis (P ＜ 0.01),and distant metastasis (P ＜ 0.01).CONCLUSION PRR11 is overexpressed in GC,which is associated with the occurrence,progression,and prognosis of GC.These data indicate that PRR11 can be used as an important biomarker to estimate the progression and prognosis of GC.