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以成纤维细胞为基因载体细胞，建立。干扰素（ＩＦＮ－α）基因疗法的实验模型，并动态观察实验动物体内ＩＦＮ－α的分泌水平。将６８２ｂｐ的全序列入ＩＦＮ－αｃＤＮＡ插入到携有Ｎｅｏ￣Ｒ基因的表达载体ＢＭＧＮｅｏ的ＸｈｏＩ位点上，对此重组表达载体（ＢＭＧＮｅｏ－ＩＦＮ－α）进行了限制性酶切鉴定。用磷酸钙共沉淀法将ＢＭＧＮｅｏ－ＩＦＮ－α转入ＮＩＨ３Ｔ３成纤维细胞中，通过Ｇ４１８抗性筛选、有限稀释和检测上清中ＩＦＮ－α生物活性，从１４株阳性克隆中筛选到一株高分泌ＩＦＮ－α达１０２４Ｕ／ｍｌ的克隆株（ＮＩＨ３Ｔ３－ＩＦＮ－α￣＋）。将此＊阳性克隆细胞体外大量扩增，包裹入胶原，然后埋植入小鼠腹腔内，于１２小时到１５天可从小鼠血清中检测出ＩＦＮ－α，以７２小时的分泌水平最高，表明成纤维细胞能成功地将人ＩＦＮ－α基因导入体内并稳定表达。
Fibroblasts were used as gene carrier cells to establish. An experimental model of interferon (IFN-α) gene therapy and dynamic observation of IFN-α secretion levels in experimental animals. The 682 bp complete sequence was inserted into the IFN-α cDNA into the XhoI site of the expression vector BMGNeo carrying the Neo ̄R gene, and the recombinant expression vector (BMGNeo-IFN-α) was subjected to restriction enzyme digestion. BMGNeo-IFN-α was transfected into NIH3T3 fibroblasts by calcium phosphate co-precipitation method. A strain was screened from 14 positive clones by G418 resistance selection, limiting dilution and detection of IFN-α biological activity in the supernatant. A clonal clone (NIH3T3-IFN-α ̄+) secreting IFN-α up to 1024 U/ml. The -positive clonal cells were extensively expanded in vitro, encapsulated in collagen, and then implanted in the abdominal cavity of mice. IFN-α was detected in the serum of the mice from 12 hours to 15 days, and the secretion level was highest at 72 hours. It was shown that fibroblasts can successfully introduce the human IFN-α gene into the body and stably express it.