为了培育出高产、稳产和抗病的贝母新品种,贝母工作者进行了平贝母与伊贝的种间远缘杂交工作。但是,这种杂交产生的往往是一些未成熟的种子(植株多因病提早枯萎,致使种子发育不完全,看不到线形胚),无发芽能力。远缘杂交的这种“不亲合性”,给育种工作造成了很大的困难。1982—1983年,我们通过贝母“杂交不育”种子的组织培养,诱导出正常的种间杂交植株,为克服贝母种间杂交不育问题,开辟了一条新途径。现把试验结果,简要报道如下。材料与方法试验材料为未成熟的杂交不育果,由我所贝母研究组提供,编号为34、45、67、159、162、163、164和“黄花类型”共八种。蒴果先用70%酒精浸半分钟,再放入0.1% In order to breed new varieties of Fritillaria with high yield, stable yield and disease resistance, Fritillaria workers conducted interspecific distant hybridization with Fritillaria cirrhosa. However, this hybridization often results in some immature seeds (plants often withered earlier due to disease, resulting in incomplete seed development, can not see linear embryos), no germination ability. This incompatibility of distant hybridization has caused great difficulties in breeding work. In 1982-1983, we induced normal interspecific hybrids through the tissue culture of Fritillaria “sterile” seeds, which opened up a new way to overcome the interspecific interspecific hybridization. Now the test results, briefly reported as follows. MATERIALS AND METHODS The test material was immature hybrid sterile fruit, which was provided by our Fritillaria mellitus study group with the numbers of 34, 45, 67, 159, 162, 163, 164 and “yellow type”. Capsule first soaked with 70% alcohol for half a minute, then add 0.1%