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目的考察5-氯水杨醛缩2,4-二羟基苯酰肼对金黄色葡萄球菌的抗菌活性及体外抗氧化活性。方法采用试管法和平板法测定其对金黄色葡萄球菌最小抑菌浓度(MIC)、最小杀菌浓度(MBC),绘制杀菌曲线,并通过扫描电镜研究其作用机制,采用DPPH法测定抗氧化活性。结果该化合物对金黄色葡萄球菌的MIC为2.5 mg/m L,MBC为5 mg/m L。杀菌曲线结果表明该化合物对金黄色葡萄球菌的杀菌作用表现出明显的浓度依赖性。扫描电镜结果表明该化合物的作用机制可能与破坏菌体细胞壁、改变其通透性有关。同时该化合物具有较强的清除自由基的能力。结论 5-氯水杨醛缩2,4-二羟基苯酰肼具有显著的抗金黄色葡萄球菌作用,同时具有较好的体外抗氧化活性。
Objective To investigate the antibacterial activity and in vitro antioxidant activity of 5-chlorosalicylaldehyde-2,4-dihydroxybenzohydrazide against Staphylococcus aureus. Methods The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Staphylococcus aureus were determined by in vitro and in vitro methods. The bactericidal curves were drawn. The mechanism of action was studied by scanning electron microscopy. The antioxidant activity was determined by DPPH method. Results The MIC for this compound against Staphylococcus aureus was 2.5 mg / m L and MBC was 5 mg / m L. Bactericidal curve results show that the compound bactericidal effect of Staphylococcus aureus showed a significant concentration-dependent. Scanning electron microscopy results show that the mechanism of action of the compound may be related to the destruction of bacterial cell wall, changing its permeability. At the same time the compound has a strong ability to scavenge free radicals. Conclusion 5-Chlorosalicylaldehyde-2,4-dihydroxybenzhydrazide has significant anti-Staphylococcus aureus activity and good in vitro anti-oxidative activity.