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背景:有研究表明改构型酸性成纤维细胞生长因子是多功能生长因子,但其抗衰老作用至今尚未有报道。目的:观察改构型酸性成纤维细胞生长因子对D-半乳糖致衰老大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力的影响。方法:将Wistar大鼠采用皮下注射D-半乳糖建立衰老模型,建模成功后随机分为模型组、生理盐水对照组和改构型酸性成纤维细胞生长因子组,另设正常对照组。改构型酸性成纤维细胞生长因子组按12μg/kg剂量肌肉注射改构型酸性成纤维细胞生长因子,生理盐水对照组肌肉注射等量的生理盐水,模型组不干预。结果与结论:与正常对照组相比,模型组大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力均明显著降低(P<0.01)。改构型酸性成纤维细胞生长因子组脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力均明显高于模型组和生理盐水对照组(P<0.05)。结果提示,改构型酸性成纤维细胞生长因子能提高衰老大鼠脑组织、肝组织及红细胞膜Na+-K+-ATP酶活力和Ca2+-Mg2+-ATP酶活力,具有延缓衰老作用。
BACKGROUND: Studies have shown that the modified acidic fibroblast growth factor is a multi-functional growth factor, but its anti-aging effect has not been reported yet. OBJECTIVE: To observe the effect of modified acidic fibroblast growth factor on Na + -K + -ATPase activity and Ca2 + -Mg2 + -ATPase activity in brain, liver and erythrocyte membrane of aging rats induced by D-galactose. Methods: The aging model was established by subcutaneous injection of D-galactose in Wistar rats. After successful modeling, the rats were randomly divided into model group, normal saline control group and modified acidic fibroblast growth factor group, and another normal control group. The modified acidic fibroblast growth factor group was intramuscularly injected with modified acidic fibroblast growth factor at a dosage of 12 μg / kg, and the normal saline control group was intramuscularly injected with the same amount of saline without any intervention by the model group. RESULTS AND CONCLUSION: Compared with normal control group, Na + -K + -ATPase activity and Ca2 + -Mg2 + -ATPase activity in brain, liver and erythrocyte membrane of model group were significantly decreased (P <0.01). The activity of Na + -K + -ATPase and Ca2 + -Mg2 + -ATPase in brain tissue, liver tissue and erythrocyte membrane in the modified acidic fibroblast growth factor group were significantly higher than those in the model group and the saline control group (P <0.05). The results suggest that the modified acidic fibroblast growth factor can increase the activity of Na + -K + -ATPase and Ca2 + -Mg2 + -ATPase in brain tissue, liver tissue and erythrocyte membrane of aging rats, and has the effect of anti-aging.